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2013 Fiscal Year Final Research Report

Study of molecular mechanisms of structural change of prestin using a novel atomic force microscope combined with a patch clamp system

Research Project

  • PDF
Project/Area Number 24791739
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Otorhinolaryngology
Research InstitutionKagoshima University (2013)
Tohoku University (2012)

Principal Investigator

MURAKOSHI Michio  鹿児島大学, 大学院理工学研究科, 准教授 (70570901)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywordsタンパク質 / 走査プローブ顕微鏡 / 生物・生体工学 / 外有毛細胞 / Prestin
Research Abstract

A cell membrane potential control device was developed. Using this device, the microstructure and electrophysiological properties of prestin-expressing culture cells were investigated. A cell was successfully positioned on a micropore of the device (about 2 um in diameter) and the microstructure of the cell surface was visualized. In addition, an artificial lipid bilayer was created on the micropore, resulting in the formation of a gigaohm seal. These results suggest that it may be possible to visualize protein reconstituted in the lipid bilayer by using this device. Based on these findings, further research to visualize the conformational change of prestin will be performed.

  • Research Products

    (9 results)

All 2014 2013 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (5 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Prestin binding peptides as ligands for targeted polymersome mediated drug delivery to outer hair cells in the inner ear2012

    • Author(s)
      Surovtseva EV, Johnston AH, Zhang W, Zhang Y, Kim A, Murakoshi M, Wada H, Newman TA, Zou J, Pyykko I.
    • Journal Title

      Int J Pharm

      Volume: 424 Pages: 121-127

    • Peer Reviewed
  • [Presentation] Force spectroscopy study on transmembrane structure of the motor protein prestin expressed in the inner ear2014

    • Author(s)
      Murakoshi M, Wada H
    • Organizer
      The 4th Japan-Switzerland Workshop on Biomechanics
    • Place of Presentation
      Shima, Mie
    • Year and Date
      20140901-04
  • [Presentation] Changes in cochlear amplification by incoordination of three-row outer hair cells. Mechanics of Hearing2014

    • Author(s)
      Murakoshi M, Suzuki S, Wada H
    • Organizer
      The 12th International Worksyop
    • Place of Presentation
      Attica, Greece
    • Year and Date
      20140623-29
  • [Presentation] Evaluation of electrophysiological properties of the motor protein prestin expressed in the inner ear2013

    • Author(s)
      Ogawa Y, Murakoshi M, Wada H
    • Organizer
      The 6th East Asian Pacific Student Workshop on Nano-Biomedical Engineering
    • Place of Presentation
      Singapore
    • Year and Date
      20130323-34
  • [Presentation] 内耳に発現する膜タンパク質プレスチンの電気生理学的特性計測のための平面パッチクランプシステムの開発2013

    • Author(s)
      小川雄大, 村越道生, 和田仁
    • Organizer
      日本機械学会第25回バイオエンジニアリング講演会
    • Place of Presentation
      筑波
    • Year and Date
      20130109-11
  • [Presentation] Effects of coordinating movement of three-row outer hair cells on the cochlear amplification2013

    • Author(s)
      Murakoshi M, Wada H
    • Organizer
      Lecture in the Faculty of Science, The University of Rome Tor Vergata
    • Place of Presentation
      Rome, Italy
    • Year and Date
      2013-12-06
  • [Book] Challenges in research on auditory mechanics. (In : T. Yamaguchi (Ed.)), Nano Biomedical Engineering 20122012

    • Author(s)
      Murakoshi M, Kumano S, Ishihara K, Iida K, Hamana H, Wada H
    • Total Pages
      15-29(676)
    • Publisher
      Imperial College Press, London
  • [Remarks] 鹿児島大学研究者総覧(ホームページ等)

    • URL

      http://kuris.cc.kagoshima-u.ac.jp/615244.html

  • [Remarks] 鹿児島大学大学院理工学研究科機械工学専攻村越研究室(ホームページ等)

    • URL

      http://www.mech.kagoshima-u.ac.jp/~michio/index.html

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Published: 2015-06-25   Modified: 2018-08-28  

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