Establishment of smoking animal model and understanding of the effect of smoking on the larynx

2014 Fiscal Year Final Research Report

• Project/Area Number: 24791749
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: The University of Tokyo
• Principal Investigator: UEHA RUMI 東京大学, 医学部附属病院, 助教 (10597131)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 喫煙 / 禁煙 / タバコ / 喉頭 / 炎症 / サイトカイン

Outline of Final Research Achievements

To figure out the mechanism of smoking effect on larynx pathophysiologically, we established how to make a smoking animal model (rats), using cigarette smoke solution, and then we examined mainly tissue alterations and changes in inflammatory cytokines.
By smoking,, mucosal epithelium became thickened, and increase of inflammatory cell infiltration, submucosal collagen fibers at the glottis, Ki67+ cells in the glottic mucosa, and hyperplasia of laryngeal glands were shown. These alterations improved by long-term smoking cessation. Additionally, though laryngeal gland secretion increased transiently, that was improved by long-term smoking cessation.
The results of RT- PCR analyses of inflammatory cytokines (TNF-α, IL-1β, IL-6, CD3e), Fibronectin1,and Osteopontin supported the organization changes in larynx.

Free Research Field

喉頭・嗅覚