2014 Fiscal Year Final Research Report
Inner ear therapy with protein transduction using polyarginine
| Project/Area Number |
24791788
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | Polyarginine / protein / inner ear |
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| Outline of Final Research Achievements |
Cell-penetrating peptides (CPPs) are short sequences of amino acids that facilitate the penetration of conjugated cargos across mammalian cell membranes. We showed that a peptide consisting of nine arginines (‘9R’) effectively delivered enhanced green fluorescent protein (EGFP) into guinea pig cochleae via the round window niche without causing any deterioration in auditory or vestibular function. A second application 24 h after the first prolonged the presence of EGFP. To assess the feasibility of protein transduction using 9R-CPPs via the round window, we used ‘X-linked inhibitor of apoptosis protein’ attached to a 9R peptide (XIAP-9R). XIAP-9R treatment prior to acoustic trauma significantly reduced putative hearing loss and the number of apoptotic hair cells in the cochleae.
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| Free Research Field |
歯科薬学
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