Analysis of microbiome in the sinonasal mucosa

2014 Fiscal Year Final Research Report

• Project/Area Number: 24791808
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Showa University
• Principal Investigator: WATANABE SO 昭和大学, 医学部, 講師 (20439491)
• Research Collaborator: SUZAKI Harumi ; HINOHIRA Yasuyuki 昭和大学, 医学部, 准教授 (00238320) ; KYO Yoshiyuki
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 副鼻腔炎 / 細菌叢 / DNA / T-RFLP

Outline of Final Research Achievements

The aim of this study is to compare the difference between sinusitis patients and healthy people on microbial flora of the sinonasal mucosa at DNA level. Bacterial samples were collected from 12 sinusitis patients and 6 healthy people. Bacterial-specific 16s rRNA was amplified by PCR and then digested by restriction enzyme. T-RFLP was performed and the data was analyzed. About 20 bacterial species were contained in each sample. Normal healthy people tended to have more bacterial species compared to sinusitis patients. In normal healthy people, more number of bacterial species were considered to get involved with prevention of disease development.

Free Research Field

耳鼻咽喉科学