2013 Fiscal Year Final Research Report
Molecular functional analysis of the modified volvox-derived channelrhodopsin-1
| Project/Area Number |
24791833
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Iwate University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 眼生理 / パッチクランプ |
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| Research Abstract |
The sensitive wavelength of channelrhodopsin2 (ChR2) derived from chlamydomonas is limited under 540 nm corresoponding to blue color. On the other hands, it has been reported that the volvox derived channelrhodopsin-1 (VChR1) had a red sifted sensitivities compared to ChR2. We developed a new type of channelrhodopsin which had a broad band wavelength sensitivities by modifying the VChR1 and investigated the ion channel properties.
After establishing the cell line which expressed mVChR1 gene (HEK-mVChR1), we measuredthe photo current caused by a light stimuli. The photo current in the modified VChR1-expressing cell induced by a stimuli of blue, green, yellow and red light, which showed approximately 30 timeshigher currents compared to that of the native VChR1. In consideration to human visual spectrum, mVChR1 was useful for the purpose of restoring vision
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