Clinical prognostic value of DNA methylation in hepatoblastoma

2015 Fiscal Year Final Research Report

• Project/Area Number: 24791886
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: Hokkaido University
• Principal Investigator: Honda Shohei 北海道大学, 大学病院, 助教 (90588089)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 肝芽腫 / メチル化 / 予後予測因子

Outline of Final Research Achievements

Using genome-wide methylation analysis and bisulfite pyrosequencing with specimens from hepatoblastoma tumors, we detected four novel genes, GPR180, MST1R, OCIAD2 and PARP6, because they likely encode tumor suppressors and their hypermethylation was associated with a poor prognosis. The methylation status of the four genes was also associated with age at diagnosis, and significant association with the presence of metastatic tumors was seen in three of the four genes. Multivariate analysis revealed that the presence of metastatic tumors and GPR180 hypermethylation were independent prognostic factors affecting event-free survival.

Free Research Field

小児外科