Investigation of Pathogenesis of Omphalocele

2014 Fiscal Year Final Research Report

• Project/Area Number: 24791892
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: Juntendo University
• Principal Investigator: DOI Takashi 順天堂大学, 医学部, 准教授 (50433777)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 免疫染色 / 多焦点顕微鏡 / 酸化ストレス / カドミウム

Outline of Final Research Achievements

We firstly observed the difference between Cd-treated chick embryo group and control group by using Hematoxilin-Eosin dying method, especially focusing on the somite development. We then extracted RNA from the chick embryo and statistically analyzed target gene expression which is strongly associated with somitogenesis. We also performed immunohistochemistry by using confocal microscope to see the distribution and intensity of target protein expression. Finally, we analyzed molecular change in the chick embryo after pre-treatment of N-acetylcysteine to reduce the oxidative stress levels. We successfully reported these results in various international meeting including Annual Meeting of European Pediatric Surgeon's Association.

Free Research Field

発生学