DIRECT EFFECT OF OK-432 AND OTHER CLINICALLY USED DRUGS FOR HUMAN LYMPHANGIOMA DERIVED LYMPHATIC ENDOTHELIAL CELL

2014 Fiscal Year Final Research Report

• Project/Area Number: 24791895
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: TAKAHASHI MASATAKA 独立行政法人国立成育医療研究センター, 臓器運動器病態外科部, フェロー (10626766)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: リンパ管腫

Outline of Final Research Achievements

Lymphangioma is still a difficult disease to obtain satisfactory results. Surgical resection or sclerotherapy is the first choice for treatment. Sevaral drugs have been reported effective for lymphangioma. OK-432 and bleomycin have been used for sclerotherapy, propranolol, sildenafil, dexamethasone and rapamycin for systemic therapy. These are very recent reports. Recently, we established a method to obtain primary culture of human lymphangioma derived lymphatic endothelial cell (HL-LEC) from surgically resected tissue. It became a very good material for studying lymphangioma. The purpose of this study is to understand mechanism of several drug’s ,especially OK432’s, direct action against lymphangioma using HL-LEC in a condition without immune reaction. HL-LECs were cultured with these drugs for up to 72 hours with oncentration’s described in several papers. In summary, OK432 bleomycin propranolol reduced viable HL-LEC.

Free Research Field

小児外科学