Development of small vessel graft with decellularized animal artery

2013 Fiscal Year Final Research Report

• Project/Area Number: 24791901
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: WANG Wei 東京医科歯科大学, 生体材料工学研究所, 助教 (60451944)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 血管再生 / 脱細胞

Research Abstract

In order to develop highly functional small artery for clinical transplantation, we tried decellularized animal artery in rat femoral artery model. Femoral arteries at the diameter of 0.8~1mm were harvested from SD rats and deccelularized with SDS detergent perfusion followed by MPC coating. Ultrasound wash and whole body perfusion were also tested to raise the decellulariazation efficacy. Treated samples were transplanted to SD rat femoral arteries. With SEM and immunostain imaging, we found SDS perfusion combined with Ultrasound treatment is an effective way to obtain decellularized artery. At 4 weeks post implant, samples were found all embolized. MPC somehow induced absorption, thus is not an appropriate treatment. Other reliable anti-coagulation method is needed.