Evaluation of culture cartilage stem / progenitor cells based on the innovative three-dimensional culture techniques that mimics the in vivo environment

2014 Fiscal Year Final Research Report

• Project/Area Number: 24791911
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Yokohama City University
• Principal Investigator: YABUKI Yuichiro 横浜市立大学, 医学(系)研究科(研究院), 客員研究員 (30610357)
• Research Collaborator: MAEGAWA Jiro ; KOBAYASHI Shinji ; TAKEBE Takanori ; KAMIJO Aki ; TANIGUCHI Hideki
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 軟骨幹／前駆細胞 / 三次元培養 / 自家血清 / 再生医療

Outline of Final Research Achievements

I produced perichondrocytes and serum from the provided perichondrium and blood. The perichondrium cells are culture expanded with autologous serum (AS) containing media and FBS-containing media and subjected to the analysis. The former is shorter cell doubling time in the AS use group and showed good growth. However, any of the group also was able to equally cartilage differentiation. In vivo experiments in severe immunodeficient mice showed the regeneration of the equivalent elastic cartilage-like tissue. The expanded perichondrocytes were inserted three-dimensional culture, RWV. Regenerated tissue is compared, and it was stable in shape in FBS group. But admitted the regenerated cartilage-like tissue in any of the group in the histologically, it was those immature.

Free Research Field

再生医療