2013 Fiscal Year Final Research Report
Investigation of endocytosis of beta4 integrin by epidermal keratinocytes
| Project/Area Number |
24791914
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Osaka City University |
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Principal Investigator |
OZAWA Toshiyuki 大阪市立大学, 医学(系)研究科(研究院), 講師 (50570602)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | Hemidesmosome / β4 integrin / リクルート / リサイクル |
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| Research Abstract |
The alpha6beta4 integrin and BP180 are major components of hemidesmosomes, and are molecules with a central role in signal transduction.We investigated beta4 integrin, a component of hemidesmosomes, to elucidate its endocytosis by the 4D live cell imaging technique. However, we could not observe endocytosis of beta4 integrin by this technique due to problems with the brightness of GFP. Therefore, we investigated endocytosis of BP180, which is another important component of hemidesmosomes, by the same technique. As a result, macropinocytosis was found to be the method of BP180 endocytosis.
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