2013 Fiscal Year Final Research Report
Development of efficient differentiation methods using cytokine produced by human adipose-derived mesenchymal stem cells
| Project/Area Number |
24791927
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Kinki University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 脂肪由来間葉系幹細胞 / 酸化ストレス / 神経伸張 / 再生医療 / 細胞治療 / p38 MAPキナーゼ |
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| Research Abstract |
In this study, we examined the role of oxidative stress on human adipose-derived multilineage progenitor cells (hADMPCs) in neurite outgrowth in PC12.
We found that glutathione depletion, followed by accumulation of reactive oxygen species, stimulates the expression of BMP2 and FGF2 in hADMPCs. Addition of N-acetylcysteine, a precursor of the intracellular antioxidant glutathione, suppressed the BSO-mediated upregulation of BMP2 and FGF2. Moreover, BSO treatment caused phosphorylation of p38 MAPK in hADMPCs. Inhibition of p38 MAPK was sufficient to suppress BMP2 and FGF2 expression, while this expression was significantly upregulated by overexpression of a constitutively active form of MKK6, which is an upstream molecule from p38 MAPK.
Thus, transplantation of hADMPCs into neurodegenerative lesions such as stroke and Parkinson's disease, in which the transplanted hADMPCs are exposed to oxidative stress, can be the basis for simple and safe therapies.
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[Journal Article] Hypoxia increases Gefitinib-resistant lung cancer stem cells through the activation of insulin-like growth factor 1 receptor2014
Author(s)
Murakami A, Takahashi F, Nurwidya F, Kobayashi I, Minakata K, Hashimoto M, Nara T, Kato M, Kajima K, Shimada N, Iwakami S, Moriyama M, Moriyama H, Koizumi F, Takahashi K
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Journal Title
PLOS ONE
Volume: 8(6)
Pages: e66274
Peer Reviewed
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[Journal Article] Hypoxia induces Gefitinib resistance in non-small cell lung cancer with both mutant and wild-type epidermal growth factor receptors2012
Author(s)
Minakata K, Takahashi F, Nara T, Hashimoto M, Tajima K, Murakami A, Nurwidya F, Yae S, Koizumi F, Moriyama H, Seyama K, Nishio K, Takahashi K
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Journal Title
Cancer Sci
Volume: 103(11)
Pages: 1946-54
Peer Reviewed
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