2013 Fiscal Year Final Research Report
Mechanisms underlying the monocytic cell death in sepsis and development of new treatments by pro-resolving lipid mediators
| Project/Area Number |
24791946
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 敗血症 / 高血糖 / 単球系細胞 / 炎症消退脂質 |
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| Research Abstract |
To develop an in vitro model of sepsis, monocytic cells were exposed to lipopolysaccharides and high glucose, which induced apoptosis and reduced phagocytosis. Our results indicate that endoplasmic reticulum stress is involved in sepsis, as an upstream regulatory mechanism. Resolvin D2, a lipid mediator involved in the resolution of inflammation, suppressed apoptosis, reduced C/EBP homologous protein expression, and improved phagocytosis, indicating that Resolvin D2 has therapeutic potential for sepsis. We are in the process of exhaustively analyzing the role of microRNAs in our model of sepsis by using Ion PGM next-generation sequencing, and in our preliminary experiments, we have observed changes in some microRNAs, including miR-211 and miR-204. We will further examine the potential of microRNAs for use in sepsis treatment in the future .
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