LC-ESI-MS(n) analysis of pro-resolving lipid mediators in epithelial lining fluid of ARDS

2014 Fiscal Year Final Research Report

• Project/Area Number: 24791947
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: TOKUHIRA Natsuko 京都府立医科大学, 医学(系)研究科(研究院), 助教 (60597227)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 急性肺障害 / 炎症消退脂質分子 / LC-ESI-MS(n) / レゾルビン / 気管支マイクロサンプリング法(BMS法) / ARDS

Outline of Final Research Achievements

Recent findings indicate that pro-resolving lipid mediators have a major role to regress inflammation. In this study, the lung epithelial lining fluid of acute lung injury patients were taken at bronchial micro-sampling (BMS) method, thus inflammation withdrawal lipid molecules contained was examined whether related to how the pathogenesis of acute lung injury.As a result using a micro-sampling BMS method with bronchoscope, the lung epithelial lining fluid (ELF) was collected in patients on mechanical ventilation, so as to LC-ESI-MS (n) analyzed using a commercially available resolvin by the adjustment repeatedly, it was confirmed that enables lipid analysis involved in inflammation regression by the BMS method. This leads future study to apply to disease based analysis.

Free Research Field

麻酔科学