2013 Fiscal Year Final Research Report

Role of tumor microenvironment in formation of late-onset lymphnode metastasis

Research Project

Project/Area Number	24791960
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Morphological basic dentistry
Research Institution	Osaka University
Principal Investigator	USAMI Yu 大阪大学, 歯学部附属病院, 助教 (80444579)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	口腔病理学 / 舌癌 / マクロファージ / 癌微小環境
Research Abstract	Oral cancer cells are exists with various non-cancerous stromal cells including inflammatory cells, and fibroblasts, and such stromal cells are referred as tumor microenvironment. It is thought that the cancer cell acquires some malignant phenotype through the cancer cell-stromal cell interactions in the microenvironment. To obtain the new material to evaluate the cancer cell-stromal cell interactions in the mouse model, we created the modified carcinogenesis model using chemical carcinogen. We also created the transgenic mouse that expresses fluorescent protein in the basal cells. Using this transgenic mouse in the carcinogenesis mouse model, it would be able for us to obtain the fluorescence protein expressing abnormal cells. In the immunohistochemical study using human tongue cancer tissue specimens, we revealed the up-regulation of invasion related protein in the cancer cell-macrophage direct interaction.