2013 Fiscal Year Final Research Report
Study of tooth morphogenesis from the aspect of local regulation of phosphate metabolism: An epigenomic approach.
| Project/Area Number |
24791962
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | エピジェネティクス |
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| Research Abstract |
We have performed histological and molecular experiment to uncover the distribution of epigenetic modifications, especially DNA methylation and hydroxymethylation, and related factors during mouse amelogenesis. The global increase of 5-hydroxymethylcytosine levels was observed when the ameloblast cells enter into secretory stage; then onset of differentiation into secretory stage, the formation of chromocenter was observed. Also, Dnmt1 mRNA and protein expression showed highest levels in immature dental epithelial cells, with decreasing levels at later stages of development. These results suggest that Dnmt1 may sustain the maintenance of undifferentiation status of dental epithelial cells.
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