A role of monocarboxylate transporters in osteoporosis caused by diabetics

2013 Fiscal Year Final Research Report

• Project/Area Number: 24791985
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Showa University
• Principal Investigator: YOSHIMURA Kentaro 昭和大学, 歯学部, 助教 (10585699)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 骨芽細胞 / 遺伝子発現 / BMP-2

Research Abstract

MCT1 is a ubiquitously expressed transmembrane transporter. It transports pyruvate, the terminal product of glycolysis, into mitochondria, resulting in the production of ATP. Introduction of siRNAs for MCT1 strongly suppressed the expression of alkaline phosphatase, an osteoblast differentiation marker, in mouse primary osteoblasts and C2C12 cells exposed to BMP-2. Furthermore, MCT-1 siRNAs suppressed phosphorylation/activation of Smad, a major signaling event after stimulation by BMP-2. In conclusion, MCT-1 not only transports its substrates but also up-regulates osteoblast differentiation by enhanced expression of the genes ruled BMP-2.

Research Products

• [Journal Article] Octacalcium phosphate suppresses chondrogenic differentiation of ATDC5 cells (2013)
  • Author(s): Shibuya I, Yoshimura K, Miyamoto Y, Yamada A, Takami M, Suzawa T, Suzuki D, Ikumi N, Hiura F, Anada T, Suzuki O, Kamijo R
  • Journal Title: Cell Tissue Res Volume: 352(2) Pages: 401-412
• [Presentation] Monocarboxylate transporter-1 は骨芽細胞におけるアルカリホスファターゼの発現に必要である (2013)
  • Author(s): 吉村健太郎, 宮本洋一, 星野真理江, 宮本 尚, 斎藤章大, 船登咲映, 泉田恵理, 槇 宏太郎, 山本松男, 馬場一美, 上條竜太郎
  • Organizer: 日本口腔組織培養学会設立50 周年記念学術大会
  • Place of Presentation: 東京
  • Year and Date: 20131123-24
• [Presentation] Monocarboxylate transporter-1 is required for cell death in mouse chondrocytic ATDC5 cells exposed to interleukin-1β via late phase activation of nuclear factor κB and expression of phagocyte-type NADPH oxidase (2013)
  • Author(s): 吉村健太郎
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山(第25回歯科基礎医学会賞受賞講演)
  • Year and Date: 20130920-22
• [Presentation] Roles of lysine-specific gingipain in osteoclast differentiation induced by inflammatory cytokines (2013)
  • Author(s): Akiyama T, Miyamoto Y, Yamada A, Takami M, Yoshimura K, Hoshino M, Imamura T, Kamijo R, Baba K
  • Organizer: University of Toronto Research Day
  • Place of Presentation: Toronto, Canada
  • Year and Date: 20130200
• [Presentation] Importance of proteolytic degradation of osteoprotegerin by lysine-specific gingipain in periodontal osteoclastogenesis (2012)
  • Author(s): Akiyama T, Miyamoto Y, Yamada A, Takami M, Yasuhara R, Yoshimura K, Hoshino M, Maruyama T, Mishima K, Baba K, Kamijo R
  • Organizer: ASBMR 2012 Annual Meeting
  • Place of Presentation: Minneapolis, MN, USA
  • Year and Date: 20121012-15
• [Presentation] 歯周病原菌由来リシン特異的ジンジパインはオステオプロテゲリンを優先的に分解しTNF-およびIL-1βによる破骨細胞分化を促進する (2012)
  • Author(s): 秋山智人, 宮本洋一, 山田 篤, 高見正道, 吉村健太郎, 星野真理江, 宮本 尚, 槇 宏太郎, 馬場一美, 上條竜太郎
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 郡山
  • Year and Date: 20120914-16
• [Presentation] Monocarboxylate transporter-1 はBMP-2刺激後のSmad1 のリン酸化に関与することで骨芽細胞分化を正に制御する (2012)
  • Author(s): 吉村健太郎, 宮本洋一, 星野真理江, 秋山智人, 塚崎雅之, 山田 篤, 高見正道, 須澤徹夫, 馬場一美, 上條竜太郎
  • Organizer: 第30回日本骨代謝学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20120719-21
• [Book] アンチエイジングシリーズ3 骨研究最前線~代謝・疾病のメカニズムから再生医療・創薬・リハビリ機器・機能性食品開 発まで~, 第3 編 第5 章 第3 節「骨代謝疾患から歯科治療へのアプローチ」 (2013)
  • Author(s): 吉村健太郎, 宮本洋一, 上條竜太郎
  • Total Pages: 257-171
  • Publisher: (株)エヌ.ティー.エス出版