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2013 Fiscal Year Annual Research Report

新規インフルエンザワクチン開発のための付着抗原の糖鎖修飾の基礎的研究

Research Project

Project/Area Number 24792010
Research InstitutionNihon University

Principal Investigator

CUENO Marni  日本大学, 歯学部, ポスト・ドクトラル・フェロー (20569967)

KeywordsH1N1 / 2013 H7N9 / Hemagglutinin / Influenza / OGS / salt bridge
Research Abstract

Influenza A virus HA glycoprotein which is a type I integral membrane with an ectodomain composed of a globular head and a stem region where both of these regions carry N-linked oligosaccharide chains. Moreover, HA proteins have several N-glycosylation sites, however, not all sites are conserved in the influenza HA protein since some of these sites can also be found in other eukaryotic N-glycosylated proteins. Among these N-glycosylation sites, overlapping glycosylation sequons (OGS) can be detected in a majority of the influenza HA subtypes where it is found to be highly conserved through several generations of the influenza virus. In one aspect of our study, we established the significance of certain salt bridges found within HA that may have resulted in cross species infection of the H1N1 strain and the assembly of the novel 2013 H7N9 strain. In the second aspect of our study, we designed a potential tomato-produced influenza subunit vaccine that utilizes the oral mucosa and targets the influenza OGS. We were able to show that an OGS-containing chimeric protein can be produced from a tomato callus. The chimeric protein amount collected was dependent on how long the bombarded tomato calli was incubated in sterile water. We were able to vaccinate mice with the chimeric protein with the ideal protein vaccination concentration, however, we only detected low antibody amounts. We suspect that either the protein vaccination concentration is too low to induce high antibody response or the ELISA assay we developed was not so sensitive. We are currently optimizing both conditions.

  • Research Products

    (12 results)

All 2014 2013 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (7 results)

  • [Journal Article] Role of the histone H3 lysine 9 methyltransferase Suv39h1 in maintaining Epstein–Barr virus latency in B95-8 cells.2014

    • Author(s)
      Imai K, Kamio N, Cueno ME, Saito Y, Inoue H, Saito I, Ochiai K
    • Journal Title

      FEBS J

      Volume: in press Pages: in press

    • DOI

      10.1111/febs.12768

    • Peer Reviewed
  • [Journal Article] Structural insights on the potential significance of the twin Asn-residue found at the base of the hemagglutinin 2 stalk in all influenza A H1N1 strains: a computational study with clinical implications.2013

    • Author(s)
      Cueno ME, Imai K, Ochiai K
    • Journal Title

      OMICS

      Volume: 17 Pages: 297-301

    • DOI

      10.1089/omi.2012.0107.

    • Peer Reviewed
  • [Journal Article] Structural differences between the avian and human H7N9 hemagglutinin proteins are attribute to modifications in salt bridge formation: a computational study with implications in viral evolution.2013

    • Author(s)
      Cueno ME, Imai K, Tamura M, Ochiai K
    • Journal Title

      PLOS ONE

      Volume: 8 Pages: 1-7

    • DOI

      10.1371/journal.pone.0076764.

    • Peer Reviewed
  • [Journal Article] Homology modeling study toward identifying structural properties in the HA2 B-loop that would influence the HA1 receptor-binding site.2013

    • Author(s)
      Cueno ME, Imai K, Shimizu K, Ochiai K
    • Journal Title

      J Mol Graph Model

      Volume: 44 Pages: 161-167

    • DOI

      10.1016/j.jmgm.2013.05.011.

    • Peer Reviewed
  • [Journal Article] Butyric acid retention in gingival tissue induces oxidative stress in jugular blood mitochondria.2013

    • Author(s)
      Cueno ME, Imai K, Matsukawa N, Tsukahara T, Kurita-Ochiai T, Ochiai K
    • Journal Title

      Cell Stress Chaperon

      Volume: 18 Pages: 661-665

    • DOI

      10.1007/s12192-013-0409-z.

    • Peer Reviewed
  • [Presentation] Periodontal bacterial metabolite induces rat blood apoptotic and pyroptotic caspase activities.

    • Author(s)
      Marni E. Cueno, Kuniyasu Ochiai
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀(東京)
  • [Presentation] Butyric acid-induced heme accumulation is associated with an increase in rat blood CASP1 and CASP3 activities

    • Author(s)
      Marni E. Cueno,Noriko Matsukawa, Takamitsu Tsukahara, Kuniyasu Ochiai
    • Organizer
      第17回国際フリーラジカル会議
    • Place of Presentation
      京都国際会議場(京都)
  • [Presentation] 口腔細菌のインフルエンザウイルス感染促進と重症化に及ぼす影響.

    • Author(s)
      神尾 宜昌, 今井 健一, 田村 宗明, Marni Cueno, 清水 一史, 落合 邦康
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場(兵庫)
  • [Presentation] Butyric acid-retention in the rat gingival tissue induces whole blood oxidative stress and apoptosis: A possible link to systemic diseases.

    • Author(s)
      Marni E. Cueno, Kenichi Imai, Muneaki Tamura, Kuniyasu Ochiai
    • Organizer
      第13回日本ミトコンドリア学会
    • Place of Presentation
      六本木アカデミーヒルズ(東京)
  • [Presentation] Positively-charged amino acid residues found in the sialidase domain influences -Porphyromonas gingivalis-.

    • Author(s)
      Marni E. Cueno, Noriaki Kamio, Kenichi Imai, Muneaki Tamura and Kuniyasu Ochiai
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山コンベンションセンター(岡山)
  • [Presentation] Homology modeling and structural analyses of the six Porphyromonas gingivalis Firm A variants.

    • Author(s)
      Marni E. Cueno, Keiji Nagano, Kenichi Imai, Fuminobu Yoshimura and Kuniyasu Ochiai
    • Organizer
      15th International Congress of Immunology
    • Place of Presentation
      Milano Congressi (イタリア)
  • [Presentation] Butyric acid-induced HEME increase is correlated with SIRT1 decrease in gingival tissues.

    • Author(s)
      Marni E. Cueno, Kenichi Imai, Noriko Matsukawa, Takamitsu Tsukahara and Kuniyasu Ochiai
    • Organizer
      The 20th IAGG world congressof Gerontology and Geriatrics
    • Place of Presentation
      Seoul Coex Convention Center(韓国)

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Published: 2015-05-28  

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