2013 Fiscal Year Final Research Report
Establishment of novel culturing system for salivary gland cells
| Project/Area Number |
24792131
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Chiba University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 唾液腺再生医療 / 口唇腺細胞 / Rock作用薬 |
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| Research Abstract |
Salivary gland (SG) dysfunction occurs as a result of radiation therapy for head cancer, and can cause a variety of critical oral health issues. In the present study, we demonstrated here a new culture system using rock-related agent (RRA) for SG cells. In addition to protein expression data that downstream molecules of RRA treatment associated with morphological changes were inhibited, we found that the RRA-treated cells showed SG like cell shape without fibroblastic differentiation, and their zenografts as well as the RRA-treated cells highly expressed the SG markers (amylase, aquaporin-5, and mucin). Furthermore, we assessed cytotoxicity of RRA to see whether we can use those reagents for clinical study. Karyotype and toxicity tests showed that RRA does not affect any genetic changes and pathological damages in vitro and in vivo models. These data suggest that our method would be important information for cell therapy of damaged SG in the near future.
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