Cryopreservation for The Cell Selection Using Water Channels AQPs Combination with Ultra-quick Freezing

2014 Fiscal Year Final Research Report

• Project/Area Number: 24792152
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Keio University
• Principal Investigator: YASUHIRO Kato 慶應義塾大学, 医学部, 助教 (40398780)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 幹細胞 / アクアポリン / 凍結

Outline of Final Research Achievements

Here we show that membrane damage caused by ultra-quick freezing is rescued by the expression of AQP4. We next examine if the expression of AQP4 combined with ultra-quick freezing can bes used to select cells efficiently under freezing conditions where most cells are died. Finally we propose that this simple but efficient and safe method may be applicable to the selection of mammalian cells for applications in regenerative medicine as well as cell-based functional assays or drug screening protocols.

Free Research Field

生化学