2014 Fiscal Year Final Research Report
Development of MicroRNA-sensitive Oncolytic Sindbis virus for Oral-cancer therapy
Project/Area Number |
24792175
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Surgical dentistry
|
Research Institution | Chiba University |
Principal Investigator |
SAITO KENGO 千葉大学, 医学(系)研究科(研究院), 講師 (70451755)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Keywords | 腫瘍溶解性ウイルス / microRNA / 口腔癌 / シンドビスウイルス |
Outline of Final Research Achievements |
The cellular tropisms of eukaryotic viruses are shaped by their need for entry receptors and intracellular transcription factors. Here we show that viral tropisms can also be regulated by tissue-specific microRNAs (miRNA). Since miRNA125b and miR100 are downregulated in oral cancer but highly expressed in normal tissue, we engineered a microRNA-sensitive virus containing target sites for those microRNAs in the 3′-untranslated region of an oncolytic Sindbis virus. The recombinant virus still propagated in cancer cells but could not replicate in cells expressing complementary miRNAs, suggesting a new modality of replicating viruses for cancer therapy.
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Free Research Field |
分子ウイルス学
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