2013 Fiscal Year Final Research Report
Epigenetic reprogramming of oral squamous cell cancer cells with oocyte extracts
| Project/Area Number |
24792217
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
FUKUI Yasuto 広島大学, 医歯薬保健学研究院, 助教 (90363085)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 歯学 / リプログラミング / 卵母細胞 |
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| Research Abstract |
I examined the expression of tumour suppressor gene (E-cadherin,p16,MGMT,DAP-K,MLH1) in oral squamous cell carcinoma cell line ZA using RT-PCR. MGMT,DAP-K and MLH1 were not expressed in ZA. ZA cells were added to Xenopus oocyte extracts (5,000 cells/ul extract) and incubated for 3,6,9 hours. Cells were collected and processed for RNA extraction. I examined the expression of tumour suppressor gene (MGMT,DAP-K,MLH1) in treated cells using RT-PCR. However, I did not recognize the significant difference.
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