2013 Fiscal Year Final Research Report
imaging of freezed oral canser tissue and cell with raman spectroscopy
| Project/Area Number |
24792265
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Fukui College of Health Sciences |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MIYOSHI Norio 福井大学, 医学部 医学科 腫瘍病理学領域, 助教 (40209961)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ラマン分光法 / 扁平上皮癌 / イメージング |
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| Research Abstract |
We analyzed oral squamous cell carcinoma with raman spectroscopy to obtain image of oral SCC tissue. The serial transverse sections were prepared, one sample section was used for raman analysis, the other sample section was stained with H&E to confirm pathological structure. Firstly, it was difficult to recognize the raman analyzing area on H&E staining image. Finally, we could adjust the raman imaging area to H&E image at 200-500um square size.
The images of raman spectroscopy show minute distribution of the protein and molecular structure at semi-quantitatively. The expression and distribution of protein was analyzed commonly by immunohistochemical methods so far. But our results indicate that raman spectroscopy is useful for analyzing the detailed distribution of various components only one time measurements.
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