2013 Fiscal Year Final Research Report

The elucidation of Runx2 mechanical stress response mechanism in cleidocranial dysplasia model mice.

Research Project

Project/Area Number	24792270
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Orthodontic/Pediatric dentistry
Research Institution	Tohoku University
Principal Investigator	AONUMA Tomo 東北大学, 大学病院, 医員 (70624823)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	メカニカルストレス / Runx2 / 細胞増殖 / メカノトランスダクション
Research Abstract	It has been reported that tooth movement in cleidocranial dysplasia(CCD) is delayed. Runx2, its mutation causes CCD, is an essential factor for osteoblastic differentiation and an important factor for mechanical stress. Our group confirmed delayed tooth movement and reduction of response for mechanical stress in periodontal tissue of Runx2 heterozygous mice (Runx2+/- mice), animal model of CCD. In present study, cell proliferation and osteoblastic differentiation in bone marrow stromal cells (BMSCs) derived from Runx2+/- mice during mechanical stretching were investigated. As a result, we found cell proliferation of BMSCs derived from wild-type and Runx2+/- mice and delayed promotion of osteoblastic differentiation of BMSCs after mechanical stretching compared with wild-type mice. We confirmed delayed osteoblastic differentiation on tension side of tooth movement in Runx2+/- mice in vitro.