2013 Fiscal Year Final Research Report
Craniofacial congenital anomaly in epigenome: the role of histone methyltransferase
| Project/Area Number |
24792278
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 歯科矯正 / 口唇口蓋裂 / エピジェネティック / ヒストン / メチル化 |
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| Research Abstract |
The aim of this study is to identify the role of histone methyltransferase SETDB1 during craniofacial development. I used Wnt1-Cre LoxP system to only knockdown SETDB1 in neural crest-derived cells and analyzed the phenotype and the downstream target of SETDB1. SETDB1fl/fl /Wnt1-Cre mouse exhibited embryonic lethality and showed cleft in palate. Palatal process was smaller than control, but had no defect of palatal elevation. The ability of proliferation was decreased in mutant mouse suggesting less proliferation of neural crest-derived cells may be a reason for cleft palate. Now I am in the middle of RNA sequencing.
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