Identification of factors to differentiate into periodontal ligament cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 24792326
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontal dentistry
• Research Institution: Osaka University
• Principal Investigator: OZASA Masao 大阪大学, 歯学部附属病院, 医員 (20624563)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 歯根膜 / 幹細胞

Research Abstract

Gene expression of plap-1 (Periodontal Ligament Associated Protein-1) was induced by the mineralization inducing culture in canine adipose tissue-derived stem cells. On the other hand, activation of HIF-1 alpha by hypoxic culture or treatment of reagents resulted in up-regulation of PLAP-1 expression in periodontal ligament cells. Furthermore, secreted factors from periodontal ligament cells stimulated the gene expression of periostin in adipose tissue-derived stem cells.
These results partly revealed the mechanisms that promote the differentiation of stem cells into periodontal ligament cells.

Research Products

• [Journal Article] Periodontal tissue regeneration by transplantation of adipose tissue-derived multi-lineage progenitor cells (2014)
  • Author(s): Masao Ozasa, Keigo Sawada, Tomoaki Iwayama, Satomi Yamamoto, Chiaki Morimoto, Hanayuki Okura, Akihumi Matsuyama, Hiroshi Komoda, Chun Man Lee, Yoshiki Sawa, Masahiro Kitamura, Tomoko Hashikawa, Masahide Takedachi, Shinya Murakami
  • Journal Title: Inflammation and Regeneration Volume: vol.34 Pages: 109-116
  • Peer Reviewed
• [Journal Article] Periodontal tissue regeneration by transplantation of adipose tissue-derived stem cells (2013)
  • Author(s): Masahide Takedachi, Keigo Sawada, Satomi Yamamoto, Masao Ozasa, Yoshio Shimabukuro, Masahiro Kitamura, Shinya Murakami
  • Journal Title: Journal of Oral Biosciences Volume: vol.55 Pages: 137-142
  • Peer Reviewed
• [Presentation] 低酸素状態が歯根膜細胞の PLAP-1発現に及ぼす影響 (2013)
  • Author(s): 山本智美, 竹立匡秀, 伊山舜吉, 沢田啓吾, 山羽聡子, 梶川哲宏, 小笹匡雄, 山田聡, 村上伸也
  • Organizer: 第56回春季日本歯周病学会学術大会
  • Place of Presentation: 東京都江戸川区
  • Year and Date: 2013-06-01
• [Presentation] 脂肪組織由来未分化間葉系幹細胞を用いた新規歯周組織再生療法の経時的評価 (2012)
  • Author(s): 沢田啓吾, 竹立匡秀, 小笹匡雄, 岩山智明, 野崎剛徳, 市川朋生, 前田憲一郎, 田内拓史, 三木康史, 大原廣之, 伊山舜吉, 安齋純, 永安利江, 寺嶋昭夫, 北村正博, 村上伸也
  • Organizer: 第30回日本骨代謝学会
  • Place of Presentation: 東京都新宿区
  • Year and Date: 2012-07-21
• [Presentation] 犬実験的歯周病モデルを用いた脂肪組織由来未分化間葉系幹細胞および FGF-2 の移植による歯周組織再生効果 (2012)
  • Author(s): 沢田啓吾, 竹立匡秀, 小笹匡雄, 岩山智明, 野崎剛徳, 市川朋生, 前田憲一郎, 田内拓史, 三木康史, 大原廣之, 伊山舜吉, 安齋純, 永安利江, 寺嶋昭夫, 北村正博, 村上伸也
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2012-06-12