2013 Fiscal Year Final Research Report
Improvement in DNA identification using STR on an X chromosome.
| Project/Area Number |
24792365
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Social dentistry
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | DNA鑑定 |
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| Research Abstract |
An X-chromosomal multiplex amplifying 18 STRs in one single PCR reaction was developed and optimized in this study. The multiplex system included the DXS7424, GATA172D05, HPRTB, DXS8377, GATA31E08, DXS6810, DXS7423, DXS981, DXS6795, DXS6803, DXS6789, DXS6800, DXS6809, DXS7133, DXS7132, DXS9902, DXS101 and DXS6807 loci, which are distributed over the whole X-chromosome. It was designed as a potential first option in determining recombination within the whole X chromosome in kinship testing. Allele frequencies were obtained from samples from 378 male and 175 female Japanese individuals, all unrelated. The combined power of discrimination of the 18 loci was 0.999999999999997 in females and 0.9999999992 in males. A case is presented in which this system allowed considerable efficacy in reaching a solution. The present multiplex system amplified the largest number of loci among the X-STR multiplex systems tested, indicating its potential in personal identification and determining kinship.
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