2013 Fiscal Year Final Research Report
Mechanism-based crosslinkers of acyl carrier protein dehydratase
| Project/Area Number |
24810015
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Living organism molecular science
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-08-31 – 2014-03-31
|
| Keywords | 生合成 / 脂肪酸合成酵素 / ポリケタイド合成酵素 / パンテテインアナログ / タンパク質-タンパク質相互作用 |
|---|
| Research Abstract |
We reported the design, synthesis, and application of sulfonyl 3-alkynyl pantetheinamides that can cross-link acyl carrier proteins (ACPs) with beta-hydroxy-ACP dehydratase (DH) domains. This cross-linking strategy provides potential to investigate these enzymes in their native binding states by offering easy access to mechanistically cross-linked enzyme species.
|
Research Products
(16 results)
-
-
-
[Journal Article] Trapping the dynamic acyl carrier protein in fatty acid biosynthesis2014
Author(s)
Chi Nguyen, Robert W. Haushalter, D.John Lee, Phineus R.L.Markwick, Joel Bruegger, Grace Caldara-Festin, Kara Finzel, David R.Jackson, Fumihiro Ishikawa, Bing O'Dowd, J.Andrew McCammon, Stanley J.Opella, Shiou-Chuan Tsai, Michael D.Burkart
-
Journal Title
Nature
Volume: 505
Pages: 427-431
DOI
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
