2013 Fiscal Year Final Research Report
Development of a biosensing technique using membrane proteins on cell-derived liposomes
| Project/Area Number |
24810019
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical biology
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
YAMANAKA Makoto 九州工業大学, バイオマイクロセンシング技術研究センター, 研究員 (80637455)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | 生体分子計測 / ヒト膜タンパク質 / リポソーム / バイオセンサ / GPCR |
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| Research Abstract |
We developed a novel technique to produce cell-derived liposomes which have specific membrane proteins expressed in human cells, and applied those liposomes to a QCM (quartz crystal microbalance) sensor for detecting interactions between membrane proteins and their ligands. Using this technique and gene transfection, we succeeded in producing liposomes which have Bradykinin B2 receptors, GPCRs for bradykinin, and detecting bradykinin using the sensor where the liposomes were immobilized.
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