2013 Fiscal Year Final Research Report
Role of methylation of RNA-biding protein on transcriptional repression by the long non-coding RNA
| Project/Area Number |
24810023
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Genome biology
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | 長鎖非コードRNA / RNA結合タンパク質 / 遺伝子発現制御 / メチル化 |
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| Research Abstract |
In this study, we attempted to uncover how the long non-coding RNAs (lncRNAs) regulate transcription via RNA-biding proteins. To investigate the role of arginine-methylation of RNA-biding protein TLS, we first developed a monoclonal antibody against arginine-methylated TLS. We performed RNA-biding immunoprecipitation assays using HeLa lysate and this antibody. We found that the lncRNA transcribed from cyclin D1 promoter binds to methylated TLS by RT-PCR. Our preliminary data also suggest that arginine methylation of TLS might enhance the biding to RNA oligonucleotides. These results suggest that the RNA-dependent transcription is regulated by arginine-methylation of RNA-biding protein.
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