2013 Fiscal Year Final Research Report

The analysis of angiogenesis inhibition by PEDF using collagen-like triple helical peptides

Research Project

Project/Area Number	24810026
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Living organism molecular science
Research Institution	Waseda University
Principal Investigator	MASUDA Ryo 早稲田大学, 理工学術院, 助手 (90632159)
Project Period (FY)	2012-08-31 – 2014-03-31
Keywords	PEDF / コラーゲン / ヘパラン硫酸 / 血管新生
Research Abstract	Although pigment epithelium-derived factor (PEDF) exhibits potent inhibitory activity against angiogenesis, its mechanism is unclear. Recently, our research group have identified the sequence on collagen which recognized PEDF. This sequence is also overlapped with heparan sulfate proteoglycan (HSPG)-recognizing sequence. In addition, PEDF interacts with not only collagen but also HSPG. We designed and synthesized collagen-like triple helical peptides which could specifically interact with PEDF or HSPG, and PEDF mutants which could specifically bind to collagen or HSPG. Furthermore, we optimized the condition of scratch assay as wound healing model to evaluate the effect of these compounds on angiogenesis.