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2013 Fiscal Year Final Research Report

Investigation on coordinate regulation of reproduction and related behaviors by kisspeptin neuronal system

Research Project

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Project/Area Number 24870007
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Animal physiology/Animal behavior
Research InstitutionThe University of Tokyo

Principal Investigator

KANDA Shinji  東京大学, 理学(系)研究科(研究院), 助教 (50634284)

Project Period (FY) 2012-08-31 – 2014-03-31
Keywordsキスペプチン / in situ hybridization / パッチクランプ / トランスジェニック / ノックアウト
Research Abstract

In this study, to unveil the novel neuronal circuits that are regulated by kisspeptin neurons in vertebrates, I visualized kisspeptin receptor-expressing neurons with GFP, and performed RNAseq of GFP positive neurons in medaka. It was shown that the peptidergic neurons that are suggested to be involved in feeding behavior and homeostasis are expressing kisspeptin receptor. These suggestion were finally confirmed by double labeling of GFP and mRNA of a certain neurotransmitter by in situ hybridization. Following electrophysiological examination showed that kisspepitn synthetic peptide depolarize these kisspeptin receptor expressing neurons. As peptidergic neurons such as isotocin and vasotocin neurons were shown to express kisspeptin receptor, knockout medaka lines of these transmitters by using TALEN technology. Further examination of these neural circuits will be examined by more detailed electrophysiology utilizing the generated knockout and transgenic medaka lines.

  • Research Products

    (25 results)

All 2014 2013 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (20 results) (of which Invited: 5 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Whole brain-pituitary in vitro preparation of the transgenic medaka (Oryzias latipes) as a tool for analyzing the differential regulatory mechanisms of LH and FSH release2014

    • Author(s)
      Karigo, T., Aikawa, M., Kondo, C., Abe, H., Kanda, S. and Oka, Y.
    • Journal Title

      Endocrinology

      Volume: Vol. 155 Pages: 536-547

    • Peer Reviewed
  • [Journal Article] Anatomical distribution of sex steroid hormone receptors in the brain of female medaka2013

    • Author(s)
      Zempo, B., Kanda, S., Okubo, K., Akazome, Y. and Oka, Y.
    • Journal Title

      J Comp Neurol

      Volume: Vol. 521 Pages: 1760-1780

    • Peer Reviewed
  • [Journal Article] Neuroanatomical evidence that kisspeptin directly regulates isotocin and vasotocin neurons2013

    • Author(s)
      Kanda, S., Akazome, Y., Mitani, Y., Okubo, K. and Oka, Y.
    • Journal Title

      PLoS One

      Volume: Vol.8 Pages: e62776

    • Peer Reviewed
  • [Presentation] 真骨魚類を用いた内分泌研究2014

    • Author(s)
      神田真司
    • Organizer
      京都大学研究大学強化促進事業百家争鳴プログラム「ムシVSサカナ」
    • Place of Presentation
      京都大学
    • Year and Date
      2014-03-15
    • Invited
  • [Presentation] 遺伝子組み換え動物を活用したキスペプチン神経系の隠された機能の探索2014

    • Author(s)
      神田真司
    • Organizer
      富山大学大学院理工学部研究部テニュアトラック制度シンポジウム
    • Place of Presentation
      富山
    • Year and Date
      2014-03-04
    • Invited
  • [Presentation] 脊椎動物キスペプチン神経系の進化と多様な機能2013

    • Author(s)
      神田真司,岡良隆
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Year and Date
      20130327-29
    • Invited
  • [Presentation] 性ステロイド感受性を示すKiss1ニューロンの電気生理学的解析2013

    • Author(s)
      長谷部政治,神田真司,島田洋之,岡良隆
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      岡山
    • Year and Date
      2013-09-27
  • [Presentation] RFRPニューロンの自発活動とGnRH1ニューロン発火抑制に関する電気生理学的解析2013

    • Author(s)
      貝瀬峻,神田真司,岡良隆
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      岡山
    • Year and Date
      2013-09-27
  • [Presentation] Functional and evolutionary diversity of vertebrate kisspeptin neuron systems2013

    • Author(s)
      Kanda, S.
    • Organizer
      Seminar in NIH
    • Place of Presentation
      Rockville
    • Year and Date
      2013-06-26
    • Invited
  • [Presentation] 脊椎動物キスペプチン神経系の進化と未知の機能2013

    • Author(s)
      神田真司,岡良隆
    • Organizer
      東京大学大気海洋研究所共同利用研究集会
    • Place of Presentation
      柏
    • Year and Date
      2013-06-22
    • Invited
  • [Presentation] 終神経ニューロン特異的なgnrh3遺伝子機能阻害2013

    • Author(s)
      小島瑠花,高橋晶子,神田真司,岡良隆
    • Organizer
      日本動物学会関東支部第65回大会
    • Place of Presentation
      東京
    • Year and Date
      2013-03-16
  • [Presentation] 遺伝子改変メダカを用いた黄体形成ホルモン(LH)放出の中枢制御機構の解析2013

    • Author(s)
      近藤千香,苅郷友美,相川雅人,神田真司,赤染康久,大久保範聡,阿部秀樹,岡良隆
    • Organizer
      日本動物学会関東支部第65回大会
    • Place of Presentation
      東京
    • Year and Date
      2013-03-16
  • [Presentation] キンギョ性フェロモン17,20β?Pによる黄体形成ホルモン(LH)分泌中枢制御系の形態学的解析2013

    • Author(s)
      吉村充史,河合喬文,善方文太郎,赤染康久,神田真司,岡良隆
    • Organizer
      日本動物学会関東支部第65回大会
    • Place of Presentation
      東京
    • Year and Date
      2013-03-16
  • [Presentation] Steroid sensitive hypothalamic Kiss1 neurons in medaka do not co-express NKB or DYN2012

    • Author(s)
      Zempo, B., Kanda, S., Akazome, Y., Oka, Y.
    • Organizer
      2nd world congress of kisspeptin
    • Place of Presentation
      Tokyo
    • Year and Date
      20121106-09
  • [Presentation] Generation of kiss1 knockout medaka using efficient new genetic tools, TALENs2012

    • Author(s)
      Takahashi, A., Kanda, S., Akazome, Y., Oka, Y.
    • Organizer
      2nd world congress of kisspeptin
    • Place of Presentation
      Tokyo
    • Year and Date
      20121106-09
  • [Presentation] Electrophysiological and morphological analysis of Kiss1 neurons in trasgenic medaka, Oryzias lapes2012

    • Author(s)
      Shimada, H., Kanda, S., Akazome, Y., Abe, H., Okubo, K., Oka, Y.
    • Organizer
      2nd world congress of kisspeptin
    • Place of Presentation
      Tokyo
    • Year and Date
      20121106-09
  • [Presentation] Embryonic expression and function of kisspeptins and their receptors in a teleost fish, medaka2012

    • Author(s)
      Okubo, K., Hodne, K., Kanda, S., Shimada, H., Oka, Y., Welzien, F.A.
    • Organizer
      2nd world congress of kisspeptin
    • Place of Presentation
      Tokyo
    • Year and Date
      20121106-09
  • [Presentation] Inhibitory regulation of gnrh3 neurons by rfrp neurons in medaka2012

    • Author(s)
      Nakane, R., Kanda, S., Oka, Y.
    • Organizer
      2nd world congress of kisspeptin
    • Place of Presentation
      Tokyo
    • Year and Date
      20121106-09
  • [Presentation] Effects of kisspeptins on LH secretion and GnRH1 neuronal activities in goldfish and medaka2012

    • Author(s)
      Karigo, T., Uenoyama, Y., Oishi, S., Fujii, N., Mitani, Y., Kanda, S., Oka, Y.
    • Organizer
      2nd world congress of kisspeptin
    • Place of Presentation
      Tokyo
    • Year and Date
      20121106-09
  • [Presentation] gpr54-1-EGFP transgenic medaka - a new approach towards the understanding of novel functions of kisspeptin neurons2012

    • Author(s)
      Kanda, S., Nakajo, M., Lee, J., Oka, Y.
    • Organizer
      2nd world congress of kisspeptin
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      20121106-09
  • [Presentation] Direct regulation of kisspeptin system on vasotocin and isotocin neurons2012

    • Author(s)
      Kanda, S., Oka, Y.
    • Organizer
      Society for Neuroscience
    • Place of Presentation
      New Orleans, LA
    • Year and Date
      20121013-17
  • [Presentation] 絶食によるメダカ視床下部NPYニューロンのNPY mRNA発現変動2012

    • Author(s)
      北原翔一,神田真司,岡良隆
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪
    • Year and Date
      20120913-15
  • [Presentation] キスペプチン受容体発現ニューロンのGFP標識による未知のキスペプチン機能の探索2012

    • Author(s)
      神田真司,李惇馥,岡良隆
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪
    • Year and Date
      20120913-15
  • [Book] Structure, Synthesis, and Phylogeny of Kisspeptin and its Receptor. In Kisspeptin Signaling in Reproductive Biology (eds K. Alexander and S. Jeremy)2013

    • Author(s)
      Kanda, S. and Oka, Y.
    • Total Pages
      9-26
    • Publisher
      Springer Science
  • [Remarks]

    • URL

      http://www.biol.s.u-tokyo.ac.jp/users/naibunpi/kanda/

URL: 

Published: 2015-07-16  

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