Intra-complex crosstalk within a super nucleoprotein structure during initiation of chromosomal DNA replication

2013 Fiscal Year Final Research Report

• Project/Area Number: 24870021
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Kyushu University
• Principal Investigator: KAWAKAMI Hironori 九州大学, 薬学研究科(研究院), 助教 (50403952)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Keywords: 染色体複製 / ORC / 相互作用様式 / 制御経路

Research Abstract

An intra-complex crosstalk within ORC, an initiator of eukaryotic DNA replication, was analyzed extensively in vitro and in vivo. Inter-complex crosstalk pathways were also identified.

Research Products

• [Journal Article] Architecture of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 on DNA reveals similarity to DNA polymerase clamp loading complexes (2013)
  • Author(s): Sun, J., Evrin, C., Samel, S., Fernández-Cid, A., Riera, A., Kawakami, H., Stillman, B., Speck , C., and Li, H.
  • Journal Title: Nat. Struct. Mol. Biol. Volume: 20(8) Pages: 944-951
  • Peer Reviewed
• [Journal Article] 出芽酵母pre-RC形成の精製組み換え蛋白質を用いた試験管内再構成とpre-IC構成因子のリクルートメント (2013)
  • Author(s): 川上広宣
  • Journal Title: Genes Genet. Syst. Volume: 87(5)(付録)(受賞記念総説) Pages: 7
• [Presentation] 出芽酵母Orc1の構造変化特異的Orc6結合に必要な残基の同定 (2013)
  • Author(s): 川上広宣、片山勉
  • Organizer: 第22回DNA複製・組換え・修復ワークショップ
  • Place of Presentation: 仙台
  • Year and Date: 20131120-22
• [Presentation] 真核生物染色体DNA複製における最初期過程の構造生物学的・生化学的基盤 (2013)
  • Author(s): 川上広宣、片山勉、Bruce Stillman
  • Organizer: 第25回微生物シンポジウム
  • Place of Presentation: 静岡
  • Year and Date: 20130906-07
• [Presentation] 精製組み換え蛋白質を用いて再構成した出芽酵母複製前複合体(pre-RC)の染色体複製開始における機能 (2013)
  • Author(s): 川上広宣、片山勉、Bruce Stillman
  • Organizer: 平成25年度日本生化学会九州支部例会
  • Place of Presentation: 佐賀県本庄町
  • Year and Date: 20130518-19
• [Presentation] 精製組み換え蛋白質を用いて再構成した出芽酵母pre-RCが複製開始に及ぼす機能 (2012)
  • Author(s): 川上広宣、片山勉、Bruce Stillman
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20121214-16
• [Presentation] 出芽酵母pre-RC形成の精製組み換え蛋白質を用いた試験管内再構成とpre-IC構成因子のリクルートメント (2012)
  • Author(s): 川上広宣、Bruce Stillman
  • Organizer: 日本遺伝学会第84回大会ワークショップ
  • Place of Presentation: 福岡(Best Papers賞)
  • Year and Date: 20120924-26
• [Presentation] Conformational transition of a nucleoprotein complex at a yeast chromosomal replication origin revealed by cryo-electron microscopy (2012)
  • Author(s): Kawakami, H., Sun, J., Zech, J., Speck, C., Li, H., and Stillman, B.
  • Organizer: Kyushu University–Pusan University Joint Seminar
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2012-09-17
• [Remarks]
  • URL: http://bunsei.phar.kyushu-u.ac.jp/