2013 Fiscal Year Final Research Report
Structural and functional analysis of a novel iron-sulpfur protein involved in the intracellular assembly of light-harvesting antenna
| Project/Area Number |
24870030
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Kurume National College of Technology |
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Principal Investigator |
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | 鉄硫黄タンパク質 / 光合成 / X線結晶構造解析 |
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| Research Abstract |
In this study, we focused on the molecular mechanism of the intracellular assembly of supramolecular complex. Especially, we studied the structure and function of the protein, involved in the assembly of cyanobacterial light harvesting antennae complex on the thylakoid membrane. We constructed cyanobacterial Ycf34 expression plasmids and purified those proteins. We found the expressed Ycf34 holds iron-sulfur cluster in the protein. The mutation analysis indicated that each of five cysteine residues of Ycf34 involved in the holding of iron-sulfur cluster in the protein.
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