2013 Fiscal Year Final Research Report
Analysis of molecular networks that regulate pattern formation and cell behavior
| Project/Area Number |
24870035
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | JT Biohistory Research Hall |
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Principal Investigator |
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | RNA-seq / 蛍光多重in situ / RNAi |
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| Research Abstract |
This study aimed to create the analysis system to understand how pattern formation and cell behavior are mutually related during early embryogenesis of the spider Parasteatoda tepidariorum. Multiple FISH and 2-step cell labeling methods are now available in this spider system. These methods in combination with embryonic RNA interference make it possible to examine RNAi effects on expression of multiple genes simultaneously and to analyze movement of knockdown-cells compared to surrounding intact cells. Furthermore, when combined with parental RNA interference, RNA sequencing is shown to be a powerful method for searching for target genes of Hedgehog signaling that is possible to regulate both pattern formation and cell behavior in the early spider embryo.
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