2013 Fiscal Year Final Research Report
Analyses of molecular mechanism of yellow symptoms induced by Cucumber mosaic virus Y satellite RNA
| Project/Area Number |
24880005
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Hokkaido University |
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Principal Investigator |
SHIMURA Hanako 北海道大学, (連合)農学研究科(研究院), 助教 (20507230)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | ウイルス / サテライトRNA / RNAサイレンシング / 病徴誘導 |
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| Research Abstract |
Cucumber mosaic virus (CMV) Y satellite RNA (Y-sat) specifically induces a bright yellow mosaic on tobacco through RNA silencing machinery. In this study, I investigated the roles of major RNA silencing components such as DCLs and RDR6 in the Y-sat-mediated yellow induction. By using a virus-induced gene silencing strategy against DCLs and RDR, I found that the accumulation levels of CMV RNAs, Y-sat, DCL2, DCL4 and RDR6 mRNAs changed in a complicated manner interfering with each other. Based on transcript analyses, it was found that DCL4 was more important than DCL2 in affecting the accumulation levels of CMV and Y-sat and thus to generate 21-nt siRNAs of Y-sat. It was also found that Y-sat siRNA levels were positively correlated with the degree of yellowing. In addition, the yellow induction by Y-sat was apparently independent of RDR6, but RDR6 indirectly affects the yellow symptoms because decreased levels of RDR6 enhanced Y-sat siRNA levels and eventually enhanced yellowing.
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[Journal Article] Infection dynamics in viral spread and interference under the synergism between Cucumber mosaic virus and Turnip mosaic virus2012
Author(s)
Takeshita M, Koizumi E, Noguchi M, Sueda K, Shimura H, Ishikawa N, Matsuura H, Ohshima K, Natsuaki T, Kuwata S, Furuya N, Tsuchiya K, Masuta C
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Journal Title
Mol. Plant Microbe Interact
Volume: 25
Pages: 18-27
Peer Reviewed
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