Identification and functional analysis of small RNAs packaged into influenza virion

2014 Fiscal Year Final Research Report

• Project/Area Number: 24880014
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied veterinary science
• Research Institution: The University of Tokyo
• Principal Investigator: MURAKAMI Shin 東京大学, 農学生命科学研究科, 特任助教 (10636757)
• Project Period (FY): 2012-08-31 – 2015-03-31
• Keywords: インフルエンザ

Outline of Final Research Achievements

It is not known whether the various small RNAs, such as small RNA derived from host cells are incorporated in influenza A virus particles. Small RNAs which were extracted from viral particles grown in embryonated chicken eggs or in Vero cells were analyzed using the next generation sequencers. As a result, host-derived tRNA and viral genomic RNA fragments were detected. We established a MDCK cells stably expressing the RNA fragment of HA segment (MDCK-5’ HA) and examined viral growth in the cells. Viral growth kinetics in MDCK-5’ HA were similar to those in wild-type MDCK cells. The expression level and localization in virus-infected cells may differ from that in the MDCK-5’ HA cells. It is necessary to prepare a cells expressing amount increased viral genome fragments and to analyze in such cells.

Free Research Field

ウイルス学