2013 Fiscal Year Final Research Report
Examination of somatic cell reprogramming efficiency with plant gene
| Project/Area Number |
24880023
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
SEMI Katsunori 京都大学, iPS細胞研究所, 研究員 (90633058)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | 体細胞初期化 / iPS細胞 / DNAメチル化 / 植物遺伝子 |
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| Research Abstract |
The global epigenetic alteration is induced by the expression of reprogramming factors during the somatic cell reprogramming. To understand of genome-wide epigenetic alteration, it is useful for the elucidation of the reprogramming mechanism. To analyze the genome-wide DNA methylation during the somatic cell reprogramming process, we performed DNA methylation analysis by RRBS methods. This result showed tumor cells gained de novo DNA methylation at ESC-methylated region, whereas kidney-methylated region retained their original methylation status. And these tumor cells have no genetic mutation at oncogenes. These results suggest that incomplete reprogramming can induce the tumor cell by the epigenetic abnormal alteration. Further, we performed forced expression of reprogramming factors with epigenetic modifier which derived from plant gene, but efficiency was not dramatically improved.
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[Journal Article] Premature termination of reprogramming in vivo leads to cancer development through altered epigenetic regulation2014
Author(s)
Ohnishi K, Semi K, Yamamoto T, Shimizu M, Tanaka A, Mitsunaga K, Okita K, Osafune K, Arioka Y, Maeda T, Soejima H, Moriwaki H, Yamanaka S, Woltjen K, Yamada Y
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Journal Title
Cell
Volume: 156(4)
Pages: 663-77
DOI
Peer Reviewed
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[Journal Article] EWS/ATF1 expression induces sarcomas from neural crest-derived cells in mice2013
Author(s)
Yamada K, Ohno T, Aoki H, Semi K, Watanabe A, Moritake H, Shiozawa S, Kunisada T, Kobayashi Y, Toguchida J, Shimizu K, Hara A, Yamada Y
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Journal Title
J Clin Invest
Volume: 123(2)
Pages: 600-10
DOI
Peer Reviewed
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[Journal Article] Treatment of a mouse model of spinal cord injury by transplantation of human induced pluripotent stem cell - derived long - term self - renewing neuroepithelial - like stem cells2012
Author(s)
Fujimoto Y, Abematsu M, Falk A, Tsujimura K, Sanosaka T, Juliandi B, Semi K, Namihira M, Komiya S, Smith A, Nakashima K
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Journal Title
Stem Cells
Volume: 30(6)
Pages: 1163-73
DOI
Peer Reviewed
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[Presentation] Premature termination of reprogramming in vivo leads to cancer development through altered epigenetic regulation2014
Author(s)
Ohnishi K, Semi K, Yamamoto T, Shimizu M, Tanaka A, Mitsunaga K, Okita K, Osafune K, Arioka Y, Maeda T, Soejima H, Moriwaki H, Yamanaka S, Woltjen K, Yamada Y
Organizer
ISSCR
Place of Presentation
Canada, Vancouver
Year and Date
20140618-21
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