2013 Fiscal Year Final Research Report
Identification of genetic factors affect the success or failure of the transformation in barley
| Project/Area Number |
24880025
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | 植物ホルモン / 再分化 / マイクロアレイ / オオムギ / 形質転換 |
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| Research Abstract |
We explored the genomic regions required for genetic transformation by using a mapping populations generated from barley cv. Golden Promise, which was a reliable cultivar for transformation. As a result of this, the partial region of chromosome No. 1H, No. 2 H, No. 3H, No. 6 H and No. 7 H were estimated as the positions of candidate genes. Then we isolated 4308 genes which expression patterns were specific in Golden Promise by a comprehensive gene expression analysis using microarray. In the analysis of phytohormone, we identified that auxin showed a positive effect in regeneration of barley callus, although abscisic acid and salicylic acid showed negative.
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