2013 Fiscal Year Final Research Report
Research on the ecology of symbiotic microbes of platypodid beetles disclosed by next-generation sequencing technology
| Project/Area Number |
24880034
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Forest science
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
ENDO Rikiya 独立行政法人理化学研究所, バイオリソースセンター, 協力研究員 (90634494)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | ナラ枯れ / カシノナガキクイムシ / 養菌性キクイムシ / 次世代シーケンサー / 酵母生態 / メタゲノム / 太平洋型 |
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| Research Abstract |
I disclosed the whole microbiome on the beetle galleries of a platypodid beetle ('pacific type') that vectors oak wilt disease.
Bait logs of Quercus serrata that attacked by 'pacific type' were collected and broken into small pieces including pupal chamber (n=2) or lateral gallery (n=4). Microbiome on the beetle galleries was analysed by culture dependent and/or non-dependent methods.
Population analysis by dilution plate technique (culture dependent) revealed that fungal population on the galleries of 'pacific type' was quite similar to that of Platypus quercivorus, a vector of Japanese Oak Wilt. Also, Actinomyces sp. was detected from all 6 samples tested by the microbiome analysis using a next generation sequencer (culture non-dependent), indicating that it is likely an important symbiont of 'pacific type'.
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