2013 Fiscal Year Final Research Report
Molecular mechanisms of the cellular coupling in the SCN during postnatal development
| Project/Area Number |
24890001
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Hokkaido University |
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Principal Investigator |
ONO Daisuke 北海道大学, 医学(系)研究科(研究院), 特任助教 (30634224)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | 概日リズム / 視交叉上核 / 時計遺伝子 / 生後発達 / 光イメージング |
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| Research Abstract |
We found that Cry1 and Cry2 are not essential for the circadian rhythm generation in the SCN cells, and are necessary for the development of intercellular networks in the adult SCN (Ono et al Nat. Commun. 2013). We also found that neonatal exposure to constant light compensates CRY functions for the disruption of circadian behavioral rhythms under constant dark (Ono et al PLOS ONE. 2013).
In order to understand the role of VIP signaling in the CryDKO SCN during development, CryDKO mice were bred with VIP receptor (VPAC2) KO mice. We measured PER2::LUC bioluminescence in the SCN from CryDKO-VPAC2KO mice.
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[Journal Article] Topological specificity and hierarchical network of the circadian calcium rhythm in the suprachiasmatic nucleus2012
Author(s)
3, Enoki, R., Kuroda, S., Ono, D., Hasan, M. T., Ueda, T., Honma, S., and Honma, K
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Journal Title
Natl. Acad. Sci
Volume: 109
Pages: 21498-21503
DOI
Peer Reviewed
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[Journal Article] Single - cell resolution fluorescence imaging of circadian rhythms detected with a Nipkow spinning disk confocal system2012
Author(s)
4, Enoki, R., Ono, D., Hasan, M. T., Honma, S., and Honma, K
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Journal Title
J. Neurosci. Mehtods
Volume: 207
Pages: 72-79
DOI
Peer Reviewed
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[Journal Article] JNK regulates the photic response of the mammalian circadian clock2012
Author(s)
5, Yoshitane H, Honma S, Imamura K, Nakajima H, Nishide SY, Ono D, Kiyota H, Shinozaki N, Matsuki H, Wada N, Doi H, Hamada T, Honma K, Fukada Y
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Journal Title
EMBO Rep
Volume: 13
Pages: 455-461
DOI
Peer Reviewed
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[Journal Article] De novo synthesis of PERIOD initiates circadian oscillation in cultured mouse suprachiasmatic nucleus after prolonged inhibition of protein synthesis by cycloheximide2012
Author(s)
6, Nishide, S. Y., Ono, D., Yamada, Y., Honma, S., and Honma, K
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Journal Title
Eur. J. Neurosci
Volume: 35
Pages: 291-299
DOI
Peer Reviewed
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