Elucidation of the diversity of target genes in GATA1

2013 Fiscal Year Final Research Report

• Project/Area Number: 24890015
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Tohoku University
• Principal Investigator: KANEKO Hiroshi 東北大学, 医学(系)研究科(研究院), 助教 (80635558)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Keywords: 遺伝子発現制御 / GATA因子 / 転写活性化部位

Research Abstract

Transcription factor GATA1 has two distinct transactivation domains (N-TAD and C-TAD). It remains to be uncovered what these multiple TADs systems confers on GATA1 function in vivo. In this study, I revealed that GATA1 target genes were classified into three groups with their dependency to TAD. In addition to this GST-pull down assay showed that GATA1 and Rb interaction was dependent to N-TAD. Because, GATA1 mutations which causes the deletion of N-TAD are found in Down's related acute megakaryoblastic leukemia(DS-AMkL) and transient myeoproliferative disorder(TMD), this result suggests that GATA1 transcriptional regulatory mechanism based on multiple TAD system is involvement to the pathology of DS-AMkL and TMD.

Research Products

• [Journal Article] Naturally occurring oncogenic GATA1 mutations with internal deletions in transient abnormal myelopoiesis in Down Syndrome (2013)
  • Author(s): Toki T, Kanezaki R, Kobayashi E, Kaneko H, et al
  • Journal Title: Blood Volume: Vol.18 Pages: 3181-4
  • URL: http://bloodjournal.hematologylibrary.org/content/121/16/3181.long
  • Peer Reviewed
• [Journal Article] N- and C- terminal transactivation domain of GATA1 protein coordinate hematopoietic program (2012)
  • Author(s): Kaneko H, Kobayashi E, Yamamoto M, Shimizu R
  • Journal Title: J. Biol. Chem Volume: Vol.287 Pages: 21439-49
  • URL: http://www.jbc.org/content/287/25/21439.full
  • Peer Reviewed