2013 Fiscal Year Final Research Report
Analysis of cytokine gene expressions with Transcription Activator-Like (TAL) protein
| Project/Area Number |
24890056
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
MATSUSHIMA Takahide 東京医科歯科大学, 医歯(薬)学総合研究科, 特任助教 (40636560)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | 炎症 / サイトカイン / TALEN / TAL / 遺伝子発現 |
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| Research Abstract |
Cytokines secreted from immune cell are central functional molecules in the immune and inflammation response. For the development of new drug and the investigation of pathogenic mechanism of immune deficient, Understanding expression mechanisms of cytokine genes is important. So, I tried the recovery of cytokine's upstream region involved transcription factors with the technique of Transcription Activator-Like Effector Nucleases (TALEN). I named this technique, "Targeted Chromatin Immunoprecipitation (T-ChIP) system". In this work, I produced the new TAL vector and investigated the T-ChIP system.
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