Role of AID as a demethylating enzymes in reactivation of chronic viral hepatits

2013 Fiscal Year Final Research Report

• Project/Area Number: 24890075
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Kanazawa University
• Principal Investigator: WAKAE kousho 金沢大学, 医学系, 助教 (70638303)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Keywords: AID/APOBEC / HBV / 脱メチル化 / 慢性感染 / B型肝炎再活性化

Research Abstract

We aimed to verify whether AID/APOBEC family could demethylate covalently-closed-circular DNA (cccDNA) of human Hepatitis B Virus (HBV). As an alternative experimental system, we utilized chicken hepatocyte cell line that stably express duck Hepatitis B Virus (DHBV) gene. The result suggested that APOBEC3G demethylate pre-core region of DHBV cccDNA.
We applied the hypothesis to Human Papilloma Viruses (HPVs), that possess circular double-strand DNA. This was supported by the experiment using a HPV16-positive cervical cell line.

Research Products

• [Journal Article] APOBEC3 deaminases induce hypermutation in human papillomavirus 16 DNA upon beta interferon stimulation (2014)
  • Author(s): Wang Z, Wakae K, Kitamura K, Aoyama S, Liu G, Koura M, Monjurul AM, Kukimoto I, Muramatsu M
  • Journal Title: J Virol. Volume: 88(2) Pages: 1308-17
  • Peer Reviewed
• [Journal Article] RNA editing of hepatitis B virus transcripts by activation-induced cytidine deaminase (2013)
  • Author(s): Liang G, Kitamura K, Wang Z, Liu G, Chowdhury S, Fu W, Koura M, Wakae K, Honjo T, Muramatsu M
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 110(6) Pages: 2246-51
  • Peer Reviewed
• [Presentation] POBEC3 hypermutates human papillomavirus (HPV) 16 genome (2013)
  • Author(s): Kousho Wakae, Satoru Aoyama, Wang Zhe, Liu Guangyan, Ahsan Monjurul, Mieko Imayasu, Miki Koura, Kouichi Kitamura, Mitsuhiro Nakamura, Satoru Kyo, Satoru Kondo, Tomokazu Yoshizaki, Iwao Kukinmoto, Tomoaki Nishiyama, Masamichi Muramatsu
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場・神戸国際展示場・神戸ポートピアホテル
  • Year and Date: 20131203-06
• [Presentation] APOBEC3はHPVゲノムにhypermutationを導入する (2013)
  • Author(s): 若江亨祥,青山慧,王哲,喜多村晃一,劉光炎,アハサン=モハメド=モンジュルル,中村充宏,京哲,柊元巌,西山智明,村松正道
  • Organizer: NGS現場の会・第三回研究会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 20130904-05
• [Presentation] APOBEC3はHPVゲノムにhypermutationを導入する (2013)
  • Author(s): 若江亨祥
  • Organizer: 第31回生化学会北陸支部大会
  • Place of Presentation: 金沢大学角間キャンパス
  • Year and Date: 2013-05-25
• [Presentation] APOBEC proteins introduce hypermutation in Human papillomavirus-16(HPV-16) viral DNA triggered by IFN stimulation (2012)
  • Author(s): 若江亨祥
  • Organizer: The 4^<th> Japan-Korea Joint Symposium on Recent Advance in Medical Science
  • Place of Presentation: 金沢大学医学部記念館
  • Year and Date: 2012-11-07