Mechanism of antiviral responses by type-II Interferon

2013 Fiscal Year Final Research Report

• Project/Area Number: 24890106
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Osaka University
• Principal Investigator: SASAI Miwa 大阪大学, 微生物病研究所, 助教 (30631551)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Keywords: 感染防御

Research Abstract

Viral infections occur at mucosal surfaces. It is important to prevent viral infection at mucosal surfaces because it is initial place. Herpes simplex virus (HSV) infect at mucosal surfaces of oral, ocular and genital. It has been reported that the protection against HSV infection are type-II interferon (IFN-gamma) dependently, especially secondary infection. However, the molecular mechanism is completely unclear. In this work, we examine the molecular mechanism of IFN-gamma mediated anti-viral immune responses, especially HSV infection. We did microarray analysis to identify gene that is involved in antiviral immune responses against HSV infection using mouse primary keratinocytes and we successfully identified the candidate molecules those are involved in suppress viral replication IFN-gamma dependently. Theses data highly assumed that this is a new mechanism of antiviral immune responses that is regulated by IFN-gamma.

Research Products

• [Journal Article] Role of mouse and human autophagy proteins in IFN-γ-induced cell-autonomous responses against Toxoplasma gondii (2014)
  • Author(s): Ohshima J, Lee Y, Sasai M, Saitoh T, Su Ma J, Kamiyama N, Matsuura Y, Pann-Ghill S, Hayashi M, Ebisu S, Takeda K, Akira S, Yamamoto M
  • Journal Title: J Immunol Volume: 192(7) Pages: 3328-35
  • DOI: 10.4049/jimmunol.1302822
  • Peer Reviewed
• [Journal Article] (2014)
  • Author(s): Ono C, Ninomiya A, Yamamoto S, Abe T, Wen X, Fukuhara T, Sasai M, Yamamoto M, Saitoh T, Satoh T, Kawai T, Ishii KJ, Akira S, Okamoto T, Matsuura Y
  • Journal Title: J Virol Volume: 88(4) Pages: 2157-67
  • DOI: 10.1128/JVI.03055-13
  • Peer Reviewed
• [Journal Article] (2013)
  • Author(s): Sasai M, Yamamoto M
  • Journal Title: Int Rev Immunol Volume: 32(2) Pages: 116-33
  • DOI: 10.3109/08830185.2013.774391
  • Peer Reviewed
• [Journal Article] (2013)
  • Author(s): Kamiyama N, Yamamoto M, Saiga H, Ma JS, Ohshima J, Machimura S, Sasai M, Kimura T, Ueda Y, Kayama H, Takeda K
  • Journal Title: PLoS One Volume: 8(2) Pages: e55800
  • DOI: 10.1371/journal.pone.0055800
  • Peer Reviewed
• [Presentation] IFN-γ依存性抗トキソプラズマ応答におけるオートファジー蛋白質の役割 (2014)
  • Author(s): 山本雅裕,大嶋淳,馬知秀,笹井美和,李英愛
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛
  • Year and Date: 2014-03-27
• [Presentation] Selective and strain-specific NFAT4 activation by the Toxoplasma gondii polymorphilic dense protein GRA6 (2013)
  • Author(s): JiSu Ma, Miwa Sasai, Kiyoshi Takeda, Masahiro Yamamoto
  • Organizer: JSI 2013
  • Place of Presentation: 幕張メッセ
  • Year and Date: 20131211-13
• [Presentation] インターフェロンγ依存性の細胞内侵入病原体排除におけるオートファジー感染タンパク質の役割の解明 (2013)
  • Author(s): 大嶋淳,笹井美和,山本雅裕,林美加子
  • Organizer: 第138回日本歯科保存学会春季学術大会
  • Place of Presentation: 福岡
  • Year and Date: 20130627-28