2013 Fiscal Year Final Research Report
Investigation and clinical application of corneal target cell-induced culture
| Project/Area Number |
24890157
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Ehime University |
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Principal Investigator |
TOMOYUKI Inoue 愛媛大学, 医学(系)研究科(研究院), 講師 (50432539)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | 角膜 / 角膜上皮 / 移植 / 再生医療 |
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| Research Abstract |
We investigated the differing characteristics of putative limbal niche cells (LNCs) and limbal stromal cells (LSCs) in the maintenance of limbal epithelial stem cells in the cornea. The colony-forming efficiency of limbal epithelial progenitor cells and the epithelial cell sheets in LNCs group was significantly better than that in the LSCs group. We developed a genetically modified line of human dermal fibroblast cells, which maintained immortalization, visualization, and eliminable characteristics. All corneal epithelial cell sheets were well stratified.
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