Analysis of relationship between periodontal tissue para-inflammation induced neuroinflammation and phagocytic activity regulation by circadian cloclk system

2013 Fiscal Year Final Research Report

• Project/Area Number: 24890161
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Kyushu University
• Principal Investigator: OKADA Ryo 九州大学, 歯学研究科(研究院), 助教 (70633105)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Keywords: 歯学 / 歯周パラインフラメーション / 脳炎症 / 食細胞 / 体内時計

Research Abstract

To elucidate the mechanism of cognitive impairment by chronic inflammatory periodontal disease, also known as para-inflammation disease, we developed animal models of periodontal disease. After the application of P. gingivalis (PG) to periodontal tissues, proinflammatory cytokines were expressed at the periodontal tissue and cerebral cortex. and intraperitoneal administration of PG caused cognitive impairment.
Microglia involved in cognitive impairment by producing proinflammatory cytokines under neuroinflammatory conditions. And recent studies showed that the abilities of microglia showed circadian rhythm. After the application of PG, proinflammatory cytokine mRNA expression levels in brain microglia and periodontal tissue macrophages showed circadian rhythm. These results suggested that circadian rhythm of proinflammatory cytokine gene expressions by periodontal tissue and brain phagocytes may affect periodontal tissue inflammation and cognitive impairment progression.

Research Products

• [Journal Article] The intrinsic microglial molecular clock controls synaptic strength via the circadian expression of cathepsin (2013)
  • Author(s): S. Yoshinori Hayashi, Satoru Koyanagi, Naoki Kusunose, Ryo Okada, Zhou Wu, Hidetoshi Tozaki-Saitoh, Kiyoharu Ukai, Shinichi Kohsaka, Kazuhide Inoue, Shigehiro Ohdo, Hiroshi Nakanishi
  • Journal Title: Sci. Rep Volume: 3巻 Pages: 2744
• [Journal Article] Differential pathways for interleukin-1βproduction activated by chromogranin A and amyloidβin microglia (2013)
  • Author(s): Zhou Wu, Li Suna, Sadayuki Hashioka, Sheng Yub, Claudia Schwab, Ryo Okada, Yoshinori Hayashi, Patrick L. McGeer, Hiroshi Nakanishi
  • Journal Title: Neurobiol. Aging Volume: 34巻 Pages: 2715-2725
• [Journal Article] Possible involvement of aiPLA2 in the phosphatidylserine-containing liposomes induced production of PGE2 and PGD2 in microglia (2013)
  • Author(s): Fumiko Takayama, Zhou Wu, Hong Mei Ma, Ryo Okada, Yoshinori Hayashi, Hiroshi Nakanishi
  • Journal Title: J. Neuroimmunol Volume: 262巻 Pages: 121-124
• [Journal Article] Diurnal spatial rearrangement of microglial processes through the rhythmic expression of P2Y12 receptors (2013)
  • Author(s): Yoshinori Hayashi, Satoru Koyanagi, Naoki Kusunose, Fumiko Takayama, Ryo Okada, Zhou Wu, Shigehiro Ohdo, Hiroshi Nakanishi
  • Journal Title: J. Neurol. Disord Volume: 1巻 Pages: 2
• [Journal Article] Brazilian green propolis suppresses the hypoxia-induced neuroinflammatory responses by inhibiting NF-κB activation in microglia (2013)
  • Author(s): Zhou Wu, Aiqin Zhu, Fumiko Takayama, Ryo Okada, Yicong Liu, Yuka Harada, Shizheng Wu, Hiroshi Nakanishi
  • Journal Title: Oxid. Med. Cell Longev Volume: 2013巻 Pages: 906726
• [Journal Article] Phosphatidylserine recognition and induction of apoptotic cell clearance by Drosophila engulfment receptor Draper (2013)
  • Author(s): Tran Thanh Tung, Kaz Nagaosa, Yu Fujita, Asana Kita, Hiroki Mori, Ryo Okada, Saori Nonaka, Yoshinobu Nakanishi
  • Journal Title: J. Biochem Volume: 153巻 Pages: 483-491
• [Journal Article] Apoptosis-dependent externalization and involvement in apoptotic cell clearance of DmCaBP1, an endoplasmic reticulum protein of Drosophila (2012)
  • Author(s): Ryo Okada, Kaz Nagaosa, Takayuki Kuraishi, Hiroshi Nakayama, Naoko Yamamoto, Yukiko Nakagawa, Naoshi Dohmae, Akiko Shiratsuchi, Yoshinobu Nakanishi
  • Journal Title: J. Biol. Chem Volume: 287巻 Pages: 3138-3146
• [Journal Article] Integrinβν-mediated phagocytosis of apoptotic cells in Drosophila embryos (2011)
  • Author(s): Kaz Nagaosa, Ryo Okada, Saori Nonaka, Kazuki Takeuchi, Yu Fujita, Tomoyuki Miyasaka, Junko Manaka, István Ando, Yoshinobu Nakanishi
  • Journal Title: J. Biol. Chem Volume: 286巻 Pages: 25770-25777
• [Remarks]
  • URL: http://www.dent.kyushu-u.ac.jp/koza/koku_jotai_seigyo/koku_kinou_bunshi/kenkyugaiyo.html