2013 Fiscal Year Final Research Report
Helicobacter bilis biliar duct infection and biliary carcinogenesis
| Project/Area Number |
24890166
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | ヘリコバクター / 感染 / 胆道発癌 / ヘリコバクタービリス |
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| Research Abstract |
A total of 115, 7-week old female Syrian golden hamsters were used. Animals were haused tree per cage with sawdust bedding under standerd laboratory condition. The Brain-Heart infusion broth containing H.bilis was adjusted to a 1x10e6 CFU of H.bilis per ml, and were used as bacterial suspensions. 1ml of the suspensions were delivered orally to each test hamster of experimentally infected group by using a sterile oral catheter three times each every other days. After dosing, the animals were normal chow and water ad libitum again. After 2, 4, 8 and 24 weeks, hamsters were euthanized and necropsied and PCR analysis for H.bilis.
In experimentally infected group, 24 animals were judged to be positive for H.bilis DNA by the PCR assays for liver and/or biliary tissue specimens. In contrast, 9 animals in control group was positive for H.bilis. Rate of Positive The difference in H.bilis DNA positivity between two groups was statistically significant (p<0.01).
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