Protective potentials of mannitol-erythropoietin against neurotoxicityof general anesthesia on immature brain.

2013 Fiscal Year Final Research Report

• Project/Area Number: 24890190
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Yokohama City University
• Principal Investigator: MAESHIMA Hanae 横浜市立大学, 医学(系)研究科(研究院), 客員研究員 (80638172)
• Research Collaborator: UCHIMOTO Kazuhiro 横浜市立大学, 医学部, 助教 (50710951) ; ANDOH Tomio 横浜市立大学, 医学研究科, 客員教授 (00193110)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Keywords: エリスロポエチン / マンニトール / 新生児 / 神経保護 / 神経細胞死 / 麻酔薬

Research Abstract

General anesthetics are shown to induce wide spread apoptosis in the developing brain. We studied if mannitol - erythropoietin (M-Epo) combined administration affords protection against anesthetic-induced neurotoxicity in neonatal rats. We found that Caspase-3 was induced in the cerebral cortex and hippocampus after 6 hour exposure to general anesthetics, meaning apoptosis increasing. Intraperitoneal administration of mannitol and Epo failed to inhibit apoptosis. In anesthetized rats, blood saturation of oxygen did not decreased, but blood concentration of carbon dioxide increased. Our results cannot suggest whether M-Epo is effective or not in inhibiting anesthetic-induced neurotoxicity in immature brain.