2013 Fiscal Year Final Research Report
The elucidation of the neurotoxic and prion-like propagative molecular mechanism of alpha synuclein and the clinical application
| Project/Area Number |
24890197
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TATEBE Harutsugu 京都府立医科大学, 医学(系)研究科(研究院), 助教 (00637027)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Keywords | アルファ・シヌクレイン / 髄液 / オリゴマー / モノマー |
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| Research Abstract |
alpha-Synuclein(a-syn) is the major pathological component of synucleinopathies including Parkinson's disease.In primary mouse hippocampal neuronal cultures, a-syn-positive synapses were colocalized with the excitatory synapse marker vesicular glutamate transporter-1.
When a-syn was evaluated with non-denaturing gels or size exclusion chromatography (SEC) columns, a-syn monomers migrated as 50- to 60-kDa proteins; however, in denaturing gels, they migrated as 15-kDa proteins. The disulfide-linked dimer and oligomer migrated slower than either the unfolded recombinant a-syn or the native a-syn in SEC.As a result,a-Syn exists predominantly as a disordered monomer in CSF.
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